Quantitation Kit Sequences Libraries

New England Biolabs offers the NEBNext Library Quant Kit for Illumina, a qPCR-based solution for accurate next generation sequencing library quantitation.

This kit offers several improvements over other library quantitation methods, including greater accuracy and higher correlation with cluster numbers, higher reproducibility, a more convenient protocol and supplied kit components. Quantitation of NGS libraries is a key step in maximizing the value and data quality of each sequencing run. Specifically for Illumina sequencing, accurate quantitationenables optimal sequence output by achieving optimal cluster densities. Further, by employing qPCR amplification, rather than electrophoresis or spectrophotometry, it becomes possible to selectively quantitate only those molecules that contain both of the adaptor sequences required for successful sequencing, further increasing the accuracy and specificity of the library quant values.