Manual Staining Kits

The best quality stains for your lab

Bio-Optica’s staining kits have many qualities, including:

Ready and easy to use

Reproducible results

Predictable cost

User safety

Limited environmental impact

For this reason our staining kits have earned ever wider acclaim in Italy and throughout the World.

Nevertheless, Bio-Optica is committed to continuous improvement of its products and their protocols for use, thanks in part to feedback from our customers, which help us uphold the highest standards of quality for our products.

For best results, please read the following guidelines.

Minimum number of tests that can be performed: The number of tests is calculated by assuming reagent consumption of 10 drops per test, which is more than sufficient to cover even medium-large sections. If you wish to use a smaller number of drops when working with small samples, you must reduce the quantity of each reagent in the same proportion in order to avoid imbalances.

Completion time: The completion time is calculated according to the duration of the individual steps of which the method consists. It does not include the time taken for de-waxing, hydrating and dehydrating the section.

Essential basic equipment: To complete the kit, you will need the following essential basic equipment:

Slide Master (code 15-MEQ0001) for horizontal slide staining

Spray bottle containing distilled water to perform the steps required by the protocol

Two series of solvents: descending for de-waxing the sections and bringing them to the water and ascending to dehydrate and diaphanize the section before mounting with coverglass.

We recommend the use of BioMount HM (codes 05-BMHM100 or 05-BMHM508) as the mounting medium.

Additional equipment: The individual instructions indicate which equipment not included in the kit, but usually available in the laboratory, is necessary to complete the kit.

Fixatives and embedding media: The protocol times were determined on histological sections of fragments fixed in formalin buffered to pH 7 with phosphate buffer and subsequently embedded in paraffin.