Advancing Neuroscience Research Through Safely Delivered Collaboration

Figure 1. (A) Activity Map displaying spontaneous spiking activity within the organoid area (outlined in white), recorded after delivery. 429 electrodes showed a firing rate > 0.1 spikes/sec. (B) Raw electrophysiological traces from four recording channels. Detected spikes are indicated by vertical lines beneath each trace. These underwent spike sorting, therefore activity from different neuronal sources within each trace are represented by different colors. Bursts of spikes are indicated by horizontal lines over the spikes, highlighting periods of rapid firing

Figure 2 (A): Network connectivity map displaying functional connections within the organoid (outlined in white) after delivery. Figure 2 (B): Firing rate trend over 10 seconds during network burst activity averaged on all active electrodes. Figure 2 (C): Raster Plot of the active electrodes displaying spikes (black dots), spike bursts (red horizontal lines) and network bursts (vertical blue lines). Figure 2 (D): Network Burst Duration. Figure 2 (E): Inter Spike Interval within Network Bursts.

Figure 3. (A) Activity Map displaying spontaneous spiking activity from three spheroids (outlined in colored circles) recorded simultaneously on the same chip following delivery. (B) Raster plot showing a representative 10 second recording of the active electrodes. Spikes from each spheroid are color coded (red el=69, blue el=90, green el=99: all with firing rates > 0.1 spikes/sec)