New Automated Cell Counter By Chemometec

The NucleoCounter® NC-200™ is a high precision cell counter utilizing state-of-the-art image cytometry.

The patented Via1-Cassette™ used for automated staining and sample handling is volume calibrated to ensure high precision and reproducibility. With user adaptable protocols and specialized assays for aggregated cells and cells on microcarriers, the NucleoCounter® NC-200™ provides an all-in-one solution for cell counting and cell viability determinations.

Visual Verification of Cell Count :

Even for simple cell count and viability assays it is essential to define the right cell population to count. The major advantage of image cytometry with the NC-200™ is the possibility to link between the images obtained and the fluorescence plots. With the intuitive accompanying NucleoView™ software it is easy to define the counting gates precisely and thereby only count the right events inside the right populations – for both total and dead cell populations.

Precise Count of Heavily Aggregated Cells :

With the aggregated cells assay the NucleoCounter® NC-200™ counts nucleated cells by fluorescent staining of the DNA. Based on a carefully developed lysis buffer system, ChemoMetec A/S offers an optimized 2-step protocol for counting and determining viability of heavily agggregated cells. In the first step, all cells are lysed and counted based on a fluorescent DNA stain. In the second step, only dead cells are counted. Within just 2 minutes, the NucleoCounter® NC-200™ will report the precise viabilty and cell count.

Optimized Counting Protocols for Cells On Microcarriers :

The NucleoCounter® NC-200™ offers a unique protocol to count and determine viability of cells grown on microcarriers without the need of detachment. With the reagents A100 and B, cells are lysed bringing the nuclei into suspension to be counted using the NC-200™ (Figure 4.). Determination of the number of dead cells is performed based on the assumption that all dead cells are free in suspension and no longer attached to the microcarrier.