KOFA's biochemical modified PA detection Kit: Study on eliminating the interference of lipid samples to the determination of prealbumin (PA)

KOFA's PA reagent kit has been through modified, updates reagent kit gain better detection effect

Prealbumin (PA) is a serum protein synthesized, rapidly transported by liver cells. It is also a negative acute reactive protein in plasma, with a molecular weight of 64KD. It is named because its swimming zone is in front of albumin when electrophoresis in pH8.6 buffer. Its physiological function is mainly to transport thyroxine and vitamin A, has the activity of thymosin, can repair tissue, promote lymphocyte maturation, improve immune function. PA is a sensitive protein type, which can accurately detect the slight changes in the liver protein synthesis ability. Prealbumin can monitor the nutritional status of patients, and then accurately reflect the actual changes of patients' organ proteins. Its half-life is short (about 2d), and changes in serum PA content can directly reflect changes in human liver cell function, providing a reliable basis for timely diagnosis of liver diseases [1-2].

After clinical feedback, the detection of PA is easily interfered by lipid blood, resulting in negative value. Lipid blood is one of the common factors leading to unqualified specimens in clinical biochemical tests, and different degrees of lipid blood will have different effects on biochemical test results [3]. Patients with hypertension, cardiovascular disease and hyperlipidemia, long-term high fat and high calorie diet {4}, clinical treatment of patients with malnutrition or wasting disease fat emulsion injection can cause fat blood specimens, therefore, is different from other unqualified specimens, often return heavy measures also cannot avoid fat blood specimens were collected [5].

KOFA continuously improved and optimized PA detection reagent to enhance the anti-interference ability of the detection reagent against lipid blood. The principle is that: through the component optimization of reagent R1, the interference of chylomicron in lipid blood sample to the detection system is effectively reduced in the first step reaction. Comparison with other manufacturers is as follows.