Live cell imaging under Inverted microscope

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Phase contrast, fluorescence

In routine laboratory work, culture living cells are available observed under phase contrast microscope, and directly record dynamic changing of live cells by microscope camera with time-lapse captured images. It is more obviously to be seen after staining. Stain live cell with fluorochorme, it can show out beautiful and bright fluorescence excited by fluorescence illumination.

Living cells is transparent when light pass through, the wavelength and amplitude almost no changes. So basic light microscope can not see the living cells without staining. In order to observe the structure of living cells, it is needed to improve the contrast of the structure through other ways. In 1930s, the Holland physicist Zernike design of the phase contrast microscope using light diffraction and disturbing. With more requirements of experiment, people expanded simple phase contrast microscope with fluorescence illumination. Those microscope is called inverted fluorescence microscope.

Professor at Jiangnan University cultivates living cells, using MSHOT Inverted fluorescence microscope MF52-LED and scientific camera MS23 at their labs, to record live cells changing and doing analysis, measure cell sizes.

MF52-LED inverted fluorescence microscope
MF52-LED inverted fluorescence microscope

MF52-LED inverted fluorescence microscope is good for fluorescence, phase contrast and bright field. The swing-in and out type condenser can achieve doing non-contaminated cultured cells observation in the high culture dish or in the cylindrical flask. Different times phase contrast objectives fit to variety magnification requirements. Modular fluorescence illumination in LED light is easy and safe to use, no need any adjustment before using, convenience to less experience users operating. With blue, green and UV fluorescence filter, it improves routinel aboratory efficiency, save space and budget.

Live cell Imaging

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