Optimized Protocols for qPCR, High-throughput Screening

Chromatrap has announced a web page that brings together protocols developed using its proprietary solid state chromatin immunoprecipitation (ChIP) technology.

The web page provides scientists with optimized ChIP methodologies for qPCR, high-throughput qPCR, sequencing, high-throughput sequencing and enzymatic chromatin shearing using Chromatrap kits. Chromatrap technology offers a quicker, easier and more efficient way of undertaking ChIP assays. The kits for qPCR use Chromatrap Protein-A or Protein-G spin columns, which contain discs of an inert, porous polymer to which protein A/G has been covalently attached.

During an assay, the chromatin/antibody complex is retained by the disc. Flushing with three buffers and an elution step are all that is required to obtain the DNA fragments of interest. Operating without the use of magnetic or agarose beads all but eliminates non-specific background and drastically reduces manual handling error and sample loss. Optimized elution buffer chemistry allows samples to be analyzed directly in qPCR without the need for DNA clean up, further reducing sample loss.