Researchers Watch Tumor Grow in Real-time

The ability to visualize and characterize the composition of a tumor in detail during its development can provide valuable insights in order to target appropriate therapeutics.

The polymer chemist Prasad Shastri and the pharmacist Jon Christensen, in collaboration with the biomedical researcher Daniel Vonwil, from the University of Freiburg have visualized and quantified the growth and composition of breast tumors over time in a living animal. The researchers published their findings in a paper in the journal PLOS ONE.

Diagnosing and treating tumors remains one of the grand challenges of medicine in the 21st century. For the translation of laboratory findings into human patients, animal models play a critical role. One way to visualize internal structures in living systems is to use light to selectively illuminate certain tissue, and analyze the reflected light to determine the structure. In such light-based imaging techniques, fluorophores — used as dyes — provide specificity and contrast, to distinguish one tissue from the other and to delineate specific tissue components.

The researchers from the University of Freiburg engineered breast cancer cells to express E2-Crimson — a protein that absorbs light in the far-red spectrum — and then grew these cells in a living system to form tumors. The scientists achieved the visualization of the tumor in real-time by illuminating the tumor expressing E2-Crimson with near infrared light followed by reconstruction of the tumor image using fluorescence molecular tomography. This enabled them to make two important findings: During the first four weeks of tumor development, the tumor volume increased not because of the increase in the tumor cells but because of the support matrix they were synthesizing. After four weeks, however, the tumor underwent a dramatic increase in the number of tumor cells. These new findings and the ability to provide visual and quantitative information on phases of tumor growth is important for selection of treatments and delivery of cancer drugs in the future.

Shastri is the Director of the Institute of Macromolecular Chemistry and core member of the cluster of excellence BIOSS Centre for Biological Signalling Studies at the University of Freiburg. Jon Christensen is a graduate student at BIOSS Centre for Biological Signalling Studies and a member of Shastris working group at the Institute of Macromolecular Chemistry. Vonwil is a postdoc in this working group.