The robust blood perfusion imaging system

The introduction of RWD RFLSI Ⅲ Laser Speckle Imaging System

RFLSI III is a robust blood perfusion imaging system. Along with advanced software, it helps researchers real-time monitor and record blood perfusion of any exposed tissues or organs for microcirculation study, to visualize the quantified data directly, shorten the experiment time, and obtain outcomes easily.

Overview

RFLSI Ⅲ is based on the LSCI (Laser Speckle Contrast Imaging) technology. With the advantages of its non-contact, high frame-rate, high spatial resolution, it can be used to observe and record blood perfusion of any exposed tissues or organs for microcirculation study.

This system is designed for pre-clinical microcirculation researches like ischemic stroke, lower limbs, mesentery, etc. Multi-Output includes blood perfusion images and videos (4.2 million pixels), quantified data for perfusion unit and vessel diameter.

Applications:

Cerebral blood perfusion monitoring

MCAO model assessment

Cortical spreading depression observation

Hind-limb ischemia research

Skin burn/skin flap transplantation

Organ microcirculation observation

Advantages

Reliable Data Measurement:

Stable laser output for reliable and consistent measurement over minutes, hours and days, accuracy up to 0.1 PU, easy to control variables.

Full-Frame HD Image & Video:

High-definition camera (full-frame 2048x2048 resolution) helps you see the blood vessel endings and opens the world of microcirculation imaging.

High Frame Rate:

A high-speed camera (up to 120 FPS) allows you to record rapid changes every second and record more details of blood vessel changes after treatment.

Quantified Visualization Data:

Full-field monitoring, quantitative analysis. Multi-output includes vascular perfusion volume, vessel diameter, vessel angle, etc., also offline state analysis available.

Guaranteed to Run Smoothly:

USB 3.0 compatible.Data acquisition and blood perfusion image with video generated rapidly without delay.

Easy Operation:

Unique non-contact technology, no need for a special dark environment. Autofocus function, easy to use, get perfusion data and video in seconds.

Successful Cases one

Journal: Nature Communication Use the laser speckle to observe changes in cerebral blood flow after lymphatic ablation, and quantitative analysis. Explore whether meningeal lymphatics are involved in clearing extravasated erythrocytes in CSF after SAH.

Successful Cases two

Journal: Diabetes Use LSCI to real-time detect regional velocity and distribution of blood flow and perfused blood vessel of sciatic nerve and foot pads, and also analyze image to better evaluate the therapeutic effect of small molecule substances on diabetes.

Successful Cases three

Journal: Shock The main purpose of the UPR is to restore ER homeostasis, and promote cell survival. It has been well established that brain I/R insult caused by transient ischemic stroke or CA/resuscitation leads to ER stress and subsequent activation of the UPR. The previous study has demonstrated that the XBP1s/HBP/O-GlcNAc axis functions in the brain, and is activated after ischemic stroke or CA (6, 8). Here, the goal was to clarify the effect of this axis on functional outcome after CA in young and aged mice.