Use Multi-Fiber Photometry to Record Neural Activities in Freely-Moving Animals

The fiber photometry system records changes in the intensity of fluorescence of neurons in specific brain regions to characterize changes in the activity of neuronal populations.

It can detect population neurons in free-moving animals for long periods of time, which is helpful to explore the correlation between neuron activity and animal behavior.

After mastering the first two chapters, you will be proficient in recording signals from free-behaving animals using a fiber photometry system. In this article, we will show some real experimental examples that will help inspire your experimental design.

The fiber photometry system used in the experimental case is as follows.

R810 Dual Color Multichannel Fiber Photometry System has two excitation light sources (410nm and 470nm). The 410 nm can be used to reflect the background noise signal, thus ensuring the acquisition of true fluorescence data.

R820 Tri-Color Multichannel Fiber Photometry System has three excitation light sources (410nm , 470nm and 560nm).The system can record signal of green fluorescence indicator like GCaMP and dLight or neurotransmitter probe and red fluorescence indicator like RCaMP, jrGECO1a or neurotransmitter probe.

Key Features and Benefits

Convenient and Efficientel

1) Up to 9 channels, suitable for multiple sites or animals

2) The software integrates data acquisition and analysis modules

3) Setting to start/end the experiment manually or automatically

4) Integrated system, fast connection

Feature-Rich Extensions

1) Supports input and output TTL signals

2) Multiple event markers can be added (external hardware/manual/track ROI tracking)

3) Built-in behavior video recording module, real-time tracking of animal tracks

4) Compatible optogenetics

Data Visualization

1) Preview mode helps to adjust experimental parameters according to test conditions

2) Real-time display of event markers allows direct observation of signal changes

3) Live display of DeltaF/F acquisition to check scale of signal changes during acquisition

4) Analysis module can directly output Df, Z-score analysis results, graphs and heat maps

High Sensitivity

1) Highly sensitive CMOS detector

2) Dual detector +TDM design

3) High stability of LED light source and laptop

4) Equipped with photobleaching device to reduce the auto fluorescence of accessories