SolisFAST® inhibitor tolerance white paper

Get a better insight into SolisFAST® qPCR range’s inhibitor tolerance capabilities.

PCR inhibitors prevent the amplification of DNA during the PCR, usually by either interacting with DNA or DNA polymerase, which may affect the sensitivity of the reaction or lead to false negative results. To avoid this, samples are generally filtered and DNA is purified. Unfortunately, it is not always possible to remove the inhibitor(s) completely. Once this happens, the inhibitors can bind to the DNA, preventing access to it. They can also reduce the availability of cofactors or interfere with their interaction with DNA polymerase. Therefore, a good inhibitor-tolerant PCR mix is very useful to avoid these kinds of inhibitor-related problems.

Solis BioDyne’s SolisFAST® DNA Polymerase is an in silico designed enzyme with fast extension rates and a programmed tolerance to inhibitory substances. Combining that with an optimized buffer, the SolisFAST® qPCR Mixes enable robust qPCR performance and accurate target detection under demanding conditions. Together with our Stability TAG technology, the mixes are not just inhibitor tolerant, but also stable at room temperature for up to six months, depending on the product.

Read the full white paper by clicking on the link below.