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RT-PCR vs RT-PCR
How to not get confused about methods?
As a starting scientist, you might have heard people mention qPCR, real-time PCR, RT-PCR and wondered if they are all the same thing or is there a difference. Turns out the answer can be yes and no. Depending on the context RT-PCR can mean qPCR or reverse transcription PCR. So when reading a scientific paper or writing one, it’s important to make sure everybody understands the abbreviations correctly.
qPCR is a quantitative method used to detect, quantify and characterize genomic fragments in real-time. Reverse transcription PCR, most commonly called RT-PCR, is a method that combines reverse transcription with PCR to detect RNA transcripts. Therefore while the abbreviations may be the same, the methods are quite different. Then there is also a third option called RT-qPCR that combines both methods and is used to quantify gene expression.
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