High-throughput genotyping

Get better results faster!

Science is continually moving toward more accurate and individual-specific approaches when it comes to developing new genotyping technologies. In light of that, we would like to discuss high-throughput genotyping, which allows for a large number of precise results to be obtained fast.

In high-throughput and automation setups, the reagents are often at higher temperatures for a long time, which can have a negative effect on enzyme activity, however all Solis Biodyne's enzymes contain the Stability TAG, thanks to which they are more stable at high temperatures and therefore very automation friendly.

SolisFAST® qPCR range offers ready-to-use probe- and dye-based qPCR mixes for fast, highly sensitive, and reproducible qPCR assays. Combining the in silico designed inhibitor tolerant SolisFAST® DNA Polymerase and an optimized buffer, the SolisFAST® qPCR Mixes enable robust qPCR performance and accurate target detection in demanding conditions. The product line offers ice-free shipping and reaction set-up.

For endpoint PCR, SolisFAST® Master Mix gives reliable results in single- and multiplex assays and has high specificity and yield. You can get results fast and there is also a dUTP/UNG-containing Mix available to prevent carryover contamination.

Another option for endpoint PCR is HOT FIREPol® Blend Master Mix Ready to Load. It offers increased yield, sensitivity, and specificity up to 5x higher fidelity. This Mix is suitable for templates up to 5 kb, has reduced primer dimer formation, and can be loaded directly on the gel after PCR. In addition, it is compatible with Illumina sequencing and customers report 52x multiplexing ability.

You can also use HOT TERMIPol® DNA Polymerase for endpoint PCR. It efficiently incorporates unconventional nucleotides and can be used for SNP detection, primer extension assay, mass array, sanger sequencing, MALDI-TOF, microarray, and DNA labeling.