3D cell obseravation with FITC,TRITC and DAPI

Allison Tan
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Fluorescence microscope observation

The Olympus BX53 is using UIS2 optical system to offer excellent precision and clarity. The entire optical path is fit to fluorescence imaging. As a standard microscope for laboratory and clinical purpose, the BX53 supports both of bright field and fluorescence in high standard. Additional, there are many accessories and objectives to satisfy today users' requirment.

 

The trinocular optimal observation head supports BX53 can connect with a variety of cameras. sCMOS camera is the latest technology digital camera designed for research level microscopes do standard fluorescence imaging. Such as FITC, TRITC and DAPI, those mostly widely used fluorescein in routine laboratory microscopy work.

 

Mshot MS60 is high resolution sCMOS camera to provide large field view, it makes user can see as large area as possible of the sample. Using with Mshot professional digital microscopy analysis software, user can combine the different fluorescence color image into one 3D image through fusion RBG.

 

We take stained cell as example

Olympus BX53 microscope

MSHOT MS60 sCMOS camera

USH100 fluorescence illumination

UPLFLN fluorescence objective 40X

0.5X C-mount adapter

Mshot digital microscopy imaging analysis software

3D Cell stained by FITC, TRITC and DAPI
3D Cell stained by FITC, TRITC and DAPI

Stained cell with triple fluorescein of FITC,TRITC and DAPI , capture separately under fluorescence microscope, then combine three different images into one, you can get a 3D cell image.

Cell stanined by FRITC

TRITC is a common fluorescein used in biological and clinical laboratory. It comes into being bright red color, The image is capture by MShot fluorescence microscope MShot MS60 sCMOS camera.

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