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【New Product Release】Sleep Deprivation System

(Includes Practical Experimental Application Cases)

Sleep Deprivation System for Laboratory Animals

The Sleep Deprivation System is a device commonly used to induce sleep deprivation in experimental animals, such as mice and rats. By periodically activating an interference rod to disturb the animals, the system effectively prevents them from entering a sleep state. This system is primarily applied in scientific research for model establishment and is widely utilized in fields such as neurophysiology, metabolic physiology, and exercise physiology.

Product Features

Touchscreen: Equipped with a 5-inch LCD capacitive touchscreen for user-friendly and convenient operation.

Interference Modes: Includes continuous, intermittent, and automatic modes. A special random mode is introduced to effectively reduce the adaptive responses of sleep-deprived animals to the environment.

Interference Rod Design: The interference rod is adjustable to two height settings (2.1 cm and 2.6 cm from the base) for precise control. It also rotates 360 degrees without dead zones to ensure safe operation, preventing injury to the animals.

Rotation Function: Offers forward, reverse, and alternating rotation modes to meet varied experimental needs.

Speed Adjustment: The device provides a wide speed range adjustable from 0.1 RPM to 20.0 RPM in increments of 0.1 RPM, allowing for fine-tuned adjustments.

Resistance Strike Mechanism: When resistance hinders movement, the device automatically reverses slightly and re-attempts until normal rotation resumes, ensuring uninterrupted experimental processes.

Supportive Accessories: Includes dedicated food and water apparatus to support long-term experimental requirements.

Activity Cylinder Design: Made from a round, transparent material to facilitate observation of animal behavior. The mouse activity cylinder measures 25 cm in diameter and 20 cm in height, while the rat activity cylinder is 40 cm in diameter and 30 cm in height, accommodating animals of different sizes.

Expandable Functional Modules:

Model:

Experimental Application Cases

1.1 Sleep Research

1.1.1 Study on the Impact of Sleep Deprivation on Cognitive Function

Background: Sleep is crucial for cognitive function. Sleep deprivation may lead to memory loss and reduced attention.

Experimental Design:

Animal Selection: Adult male mice, aged 3-4 months, weighing 25-30 grams.

Groups: Randomly divide mice into two groups: sleep deprivation group and control group.

Sleep Deprivation Method: Use the interference rod system to deprive the sleep deprivation group of sleep for 24 hours, while allowing the control group to rest normally.

Behavioral Tests: Use the Morris Water Maze to test memory, and the Y Maze to test attention.

Testing Methods:

Morris Water Maze: Assess spatial memory by recording the time and path taken by mice to find a hidden platform.

Y Maze: Test attention and short-term memory by recording time spent in new and familiar arms of the maze.

Data Analysis:

Statistical Methods:Compare test results between sleep deprivation and control groups using t-tests or ANOVA.

Graphical Representation:Use bar and line graphs to display dat

1.1.2 Study on the Impact of Sleep Deprivation on Physiological Function

Background: Sleep deprivation may affect the immune, metabolic, and cardiovascular systems.

Experimental Design:

Animal Selection: Adult male rats, aged 3-4 months, weighing 250-300 grams.

Groups: Randomly divide rats into two groups: sleep deprivation group and control group.

Sleep Deprivation Method: Use the interference rod system to deprive the sleep deprivation group of sleep for 48 hours, while allowing the control group to rest normally.

Physiological Indicators: Collect blood samples to measure white blood cell count, cortisol levels, blood glucose, and lipids.

Testing Methods:

Blood Collection: Collect blood via the tail vein at the end of the experiment and separate serum.

Biochemical Analysis: Use ELISA to measure cortisol levels and a biochemical analyzer to measure blood glucose and lipids.

Data Analysis:

Statistical Methods: Compare physiological indicators between sleep deprivation and control groups using t-tests or ANOVA.

Graphical Representation: Use bar and scatter plots to display data.

1.2 Neuroscience Research

1.2.1 Study on the Impact of Sleep Deprivation on Neuronal Activity

Background: Sleep deprivation may alter brain neuronal activity patterns.

Experimental Design:

Animal Selection: Adult male mice, aged 3-4 months, weighing 25-30 grams.

Groups: Randomly divide mice into two groups: sleep deprivation group and control group.

Sleep Deprivation Method: Use the interference rod system to deprive the sleep deprivation group of sleep for 12 hours, while allowing the control group to rest normally.

Electrophysiological Recording: Monitor neuronal activity in specific brain regions using EEG and single-cell recording techniques.

Testing Methods:

Electrode Implantation: Implant electrodes in the hippocampus or prefrontal cortex.

Data Recording: Record neuronal firing patterns with a multi-channel recording system.

Data Analysis:

Signal Processing: Analyze EEG signals using Fourier transform and other techniques.

Statistical Methods: Compare neuronal activity between sleep deprivation and control groups using t-tests or ANOVA.

Graphical Representation: Use spectrograms and firing rate graphs to display data.

1.2.2 Study on the Impact of Sleep Deprivation on Neurotransmitter Levels

Background: Sleep deprivation may influence neurotransmitter levels in the brain.

Experimental Design:

Animal Selection: Adult male rats, aged 3-4 months, weighing 250-300 grams.

Groups: Randomly divide rats into two groups: sleep deprivation group and control group.

Sleep Deprivation Method: Use the interference rod system to deprive the sleep deprivation group of sleep for 24 hours, while allowing the control group to rest normally.

Neurotransmitter Detection: Collect brain tissue samples to measure levels of neurotransmitters like dopamine and serotonin.

Testing Methods:

Brain Tissue Collection: Quickly extract brain tissue and isolate target regions after the experiment.

High-Performance Liquid Chromatography (HPLC): Use HPLC to measure neurotransmitter levels.

Data Analysis:

Statistical Methods: Compare neurotransmitter levels between sleep deprivation and control groups using t-tests or ANOVA.

Graphical Representation: Use bar and scatter plots to display data.

1.3 Behavioral Research

1.3.1 Study on the Impact of Sleep Deprivation on Emotions and Behavior

Background: Sleep deprivation may lead to emotional instability and abnormal behavior.

Experimental Design:

Animal Selection: Adult male mice, aged 3-4 months, weighing 25-30 grams.

Groups: Randomly divide mice into two groups: sleep deprivation group and control group.

Sleep Deprivation Method: Use the interference rod system to deprive the sleep deprivation group of sleep for 24 hours, while allowing the control group to rest normally.

Behavioral Tests: Use the Open Field Test to assess anxiety levels and the Forced Swim Test to evaluate depressive behavior.

Testing Methods:

Open Field Test: Record time and location of mouse activity in the open field to assess anxiety.

Forced Swim Test: Record immobility time in water to assess depressive behavior.

Data Analysis:

Statistical Methods: Compare behavioral performance between sleep deprivation and control groups using t-tests or ANOVA.

Graphical Representation: Use bar and line graphs to display data.

Summary

The Interference Rod Sleep Deprivation System has extensive applications in sleep, neuroscience, and behavioral research. Through carefully designed experiments and rigorous data analysis, the system enables in-depth exploration of the effects of sleep deprivation on animal physiology and behavior, providing valuable scientific insights for related research fields.

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Details

  • Si Zhuan Nan Lu, Song Jiang Qu, Shang Hai Shi, China, 201619
  • Shanghai TOW Intelligent Technology

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